Dataset: interferENZY_example
Moving average filter applied with an interval of 9, centered on each timepoint.
Sampled 7 equally-distanced points to obtain 5 modified sets of coordinates, taking maximum product obtained as reference.

-Global Fitting, Full Dataset 

Instant 	 Km 	 SE 	 V 	 SE 
1	1.094731472368722	0.01695331470454418	2.735796285132495	0.03205170152253446
2	1.088062851590248	0.0239061504850768	2.741470015364979	0.04664778336578065
3	1.102939443123977	0.04402941166950015	2.788234922908052	0.08858346627525879

Instant 	 R2 	 F 	 p value 	 Estimated Error Variance 
1	0.9933296980856475	4169.711042097324	0	0.00385183343794662
2	0.9894913718556801	2071.517792985318	0	0.005531168927439038
3	0.9751361895954949	627.5055504244425	2.897682094271659e-14	0.01201658842036494

Coefficient of determination (R2) 
Instant 	1	2	3
 	0.9933296980856475	0.9894913718556801	0.9751361895954949

Kendall Correlation Coefficient (K) 
Instant/S0 	1	2	3
0.01	-0.2	-0.3333333333333334	0.3333333333333334
0.1	-0.7999999999999999	-0.6666666666666667	-0.3333333333333334
0.5	-0.4	0.3333333333333334	1
1	-1	-1	-1
2.5	-1	-1	-1
5	-1	-1	-1

Final Score (0-20) 
Instant 	1	2	3
 	19.23327880443471	18.8701483554602	17.96037617718242

Best guess for apparent kinetic parameters from final score analysis: 
Instant 	 Km 	 SE 	 V 	 SE 
1	1.094731472368722	0.01695331470454418	2.735796285132495	0.03205170152253446

Best guess for apparent parameter Km is within the range covered by experimental S0 values.


Upper S0 range is lower than 2*Km (max(S0) > 2*Km).
A broader range of S0 values is recommended for accurate kinetic analysis.

-Global Fitting, initial points only (initial reaction phase analysis) 

Instant 	 Km 	 SE 	 V 	 SE 
1	1.102741803047327	0.03359858326580418	2.727367386244019	0.06058332578948279
2	1.083220957819803	0.0114520114102595	2.720331353010862	0.02151355714080499
3	1.148755911155607	0.01642787252716695	2.921549461066468	0.03258883340223503

Instant 	 R2 	 F 	 p value 	 Estimated Error Variance 
1	0.9963004867463157	1077.223319315436	5.138741697607863e-06	0.003421976060371671
2	0.9995531154866641	8946.858399949686	7.490082154504307e-08	0.0003640375790217691
3	0.99918264259302	4889.819968891326	2.505957122922453e-07	0.0006359174424326915

Comparison between results for full dataset and initial points:
No considerable time-dependent effects are observed.